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, 2004], suggesting that cell-cell communication is a likely component of the stabilization observed in this model system Consistent with the importance of homotypic (hepatocyte-hepatocyte) interactions, spheroidal aggregates have been shown to promote the formation of bile canaliculi, gap junctions, and tight junctions and the expression of E-cadherins and several differentiated functions [Chang, 1992; Landry et al, 1985; Saito et al, 1992] Similar to homotypic communication, heterotypic (hepatocyte-nonparenchymal) interactions have also been shown to improve viability and differentiated function This stabilization of hepatocyte functions has been reported for hepatocyte cocultures with both liver- and non-liver-derived cell types, and, furthermore, bene cial effects of cross-species coculture systems have also been observed [Bhatia et al, 1999] Together, these ndings suggest a highly conserved mechanism by which cocultures enhance the liver-speci c function of hepatocytes However, the precise roles of the potential regulatory factors, such as secreted signals (ie.

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, cytokines) or cell-associated signals (ie, insoluble extracellular matrix or membrane-bound molecules), in the coculture effect have not yet been clearly de ned An understanding of the mechanisms mediating the stabilization of hepatocytes in coculture would have broad implications in liver cell biology and, speci cally, would aid in the development of functional hepatic tissue constructs In addition to static culture systems, several bioreactor designs have also been developed as in vitro hepatocyte culture models Perfusion systems would facilitate enhanced nutrient delivery to hepatocytes, which are highly metabolic, and additionally, in contrast to a batch process, would enable the continuous processing of blood or serum, potentially useful for extracorporeal devices Overall, the bioreactor systems developed to date fall into the following categories: at plate, hollow ber, perfusion scaffolds, and packed beds, each with accompanying advantages and disadvantages [Allen et al, 2001] (Fig 152).

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7. Jyoti Thottam, Thank God It s Monday!, Time, January 17, 2005. 8. Occupational Outlook Handbook Engineers, United States Department of Labor, Bureau of Labor Statistics, August 4, 2006. 9. The Story of Xerography, Xerox Corporation, www.xerox.com/ downloads/usa/en/s/Storyofxerography.pdf. 10. Playmakers Part II: Play-Doh, Parents Choice Foundation, www .parents-choice.org/full_abstract.cfm art_id=236&the_page=editorials. 11. Henry Petroski, Painful Design, American Scientist 93, no. 2 (2005): 113. 12. Brad Stone and Robert Stein, Is TiVo s Time Up , Newsweek, March 20, 2006. 13. Steve Kerr, interview, 2005. 14. Ibid. 15. Mary J. Benner and Michael Tushman, Process Management and Technological Innovation: A Longitudinal Study of the Photography and Paint Industries, Johnson Graduate School, Cornell University, Ithaca, New York, 2002. 16. Barbara Ross et al., The Great Tyco Robbery, New York Daily News, September 12, 2002. 17. Steve Kerr, interview, 2005. 18. Merriam-Webster s Words of the Year 2005, Merriam-Webster, www.m-w .com/info/05words.htm.

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In particular, the combination of a at plate reactor system with sandwich culture or coculture has been illustrated to improve hepatocyte stability for long-term analysis [Bader et al, 1995; Tilles et al, 2001] Additionally, a recent three-dimensional perfusion bioreactor system has been developed based on the morphogenesis of hepatocytes into three-dimensional structures in an array of channels [Powers et al, 2002a,b] Similar to static culture.

0 as Pe(n) 0. Hence, nR = H (W ) = I (W ; W ) + H (W |W ) I (W ; W ) + n I (X n ; Y n ) + n

When Apple introduced Automator in 2004, there was speculation and confusion that Automator was nothing but a higher-level interface on AppleScript. It s easy to see how that false assumption could arise, because both technologies are used to automate work. But it s not true. Automator provides a scripting-free way to perform repetitive tasks. Those tasks can be defined in AppleScript, Cocoa, or bits of both; but in any case, Automator is not just a pretty non-programming face on AppleScript.

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